Simultaneous Determination of Dipyrone Metabolites in Rat Hypothalamus, Cerebrospinal Fluid and Plasma Samples by LC–MS/MS
Male
03 medical and health sciences
0302 clinical medicine
Tandem Mass Spectrometry
Dipyrone
Hypothalamus
Animals
Rats, Wistar
Chromatography, High Pressure Liquid
Rats
3. Good health
DOI:
10.4155/bio.13.229
Publication Date:
2013-11-01T16:04:43Z
AUTHORS (8)
ABSTRACT
After oral administration dipyrone is rapidly hydrolyzed to 4-methylaminoantipyrine, which absorbed and further metabolized 4-formylaminoantipyrine 4-aminoantipyrine, acetylated by a polymorphic N-acetyltransferase system 4-acetylaminoantipyrine. To evaluate the presence of metabolites in different rat matrices after intraperitoneal administration, an analytical method was developed validated.The four main were extracted from plasma, cerebrospinal fluid hypothalamus samples LLE prior LC-MS/MS.Standard calibration graphs for all linear (r > 0.99). The intra- inter-day precision accuracy values both inferior 15%.This simple specific studying administration.
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