Simultaneous Determination of Dipyrone Metabolites in Rat Hypothalamus, Cerebrospinal Fluid and Plasma Samples by LC–MS/MS

• DOI: 10.4155/bio.13.229
• Publication Date: 2013-11-01T16:04:43Z
• Authors: Fernando A Aguiar; David do C Malvar; Artur de LL Vaz; Leandro A Calixto; Giuliano C Clososki; Cristiane M de Ga...; Glória EP de Souza; Valquíria AP Jabor
• Keywords: Male; 03 medical and health sciences; 0302 clinical medicine; Tandem Mass Spectrometry; Dipyrone; Hypothalamus; Animals; Rats, Wistar; Chromatography, High Pressure Liquid; Rats; 3. Good health

After oral administration dipyrone is rapidly hydrolyzed to 4-methylaminoantipyrine, which absorbed and further metabolized 4-formylaminoantipyrine 4-aminoantipyrine, acetylated by a polymorphic N-acetyltransferase system 4-acetylaminoantipyrine. To evaluate the presence of metabolites in different rat matrices after intraperitoneal administration, an analytical method was developed validated.The four main were extracted from plasma, cerebrospinal fluid hypothalamus samples LLE prior LC-MS/MS.Standard calibration graphs for all linear (r > 0.99). The intra- inter-day precision accuracy values both inferior 15%.This simple specific studying administration.