Simultaneous Determination of Dipyrone Metabolites in Rat Hypothalamus, Cerebrospinal Fluid and Plasma Samples by LC–MS/MS
Male
03 medical and health sciences
0302 clinical medicine
Tandem Mass Spectrometry
Dipyrone
Hypothalamus
Animals
Rats, Wistar
Chromatography, High Pressure Liquid
Rats
3. Good health
DOI:
10.4155/bio.13.229
Publication Date:
2013-11-01T16:04:43Z
AUTHORS (8)
ABSTRACT
After oral administration dipyrone is rapidly hydrolyzed to 4-methylaminoantipyrine, which is absorbed and further metabolized to 4-formylaminoantipyrine and to 4-aminoantipyrine, which is acetylated by a polymorphic N-acetyltransferase system to 4-acetylaminoantipyrine. To evaluate the presence of dipyrone metabolites in different rat matrices after intraperitoneal administration, an analytical method was developed and validated.The four main dipyrone metabolites were extracted from plasma, cerebrospinal fluid and hypothalamus samples by LLE prior to LC-MS/MS.Standard calibration graphs for all metabolites were linear (r > 0.99). The intra- and inter-day precision and accuracy values were both inferior to 15%.This method is simple and specific for studying dipyrone metabolites after intraperitoneal administration.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (28)
CITATIONS (5)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....