Simultaneous Determination of Centchroman and Tamoxifen Along with Their Metabolites in Rat Plasma Using LC–MS/MS

• DOI: 10.4155/bio.14.253
• Publication Date: 2015-05-12T15:53:30Z
• Authors: Kanumuri Siva Ram...; Isha Taneja; Sheelendra Pratap...; Amit Tripathi; Durga Prasad Mishra; K Mahaboob Hussain; Jiaur Rahman Gayen; Shio Kumar Singh; Muhammad Wahajuddin
• Keywords: 0301 basic medicine; Spectrometry, Mass, Electrospray Ionization; 0303 health sciences; 9,10-Dimethyl-1,2-benzanthracene; Mammary Neoplasms, Experimental; Rats; 3. Good health; Rats, Sprague-Dawley; Centchroman; Tamoxifen; 03 medical and health sciences; Tandem Mass Spectrometry; Carcinogens; Animals; Metabolomics; Female; Tissue Distribution; Chromatography, Liquid

Tamoxifen and centchroman are two non-steroidal, selective estrogen receptors modulators, intended for long term therapy in the woman. Because of their wide spread use, there is a possibility of co-prescription of these agents.We studied the probable pharmacokinetic interaction between these agents in breast cancer model rats. A simple, sensitive and rapid LC-ESI-MS/MS method was developed and validated for the simultaneous determination of tamoxifen, centchroman and their active metabolites.The method was linear over a range of 0.2-200 ng/ml. All validation parameters met the acceptance criteria according to regulatory guidelines.LC-MS/MS method for determination of tamoxifen, centchroman and their metabolites was developed and validated. Results show the potential of drug-drug interaction upon co-administration these two marketed drugs.