Salivary miRNAome profiling uncovers epithelial and proliferative miRNAs with differential expression across dentition stages

• DOI: 10.4161/cc.10.19.17647
• Publication Date: 2011-10-26T15:42:20Z
• Authors: Amanda Formosa; Maria Cristina Piro; Raffaella Docimo; Paolo Maturo; Daniela Rita Mari...; Murugan Kalimutho; Giulia Sancesario; Alberto Barlattani; Gerry Melino; Eleanora Candi; Sergio Bernardini
• Keywords: Settore BIO/11 - BIOLOGIA MOLECOLARE; 0303 health sciences; Adolescent; Reverse Transcriptase Polymerase Chain Reaction; Gene Expression Profiling; 500; Infant; Dentition, Permanent; MicroRNAs; 03 medical and health sciences; Gene Expression Regulation; Child, Preschool; Humans; Tooth, Deciduous; Child; Saliva; Tooth

Saliva's ability to mirror the internal physiological environment of an organism coupled with its facile accessibility makes it attractive diagnostic medium. The finding microRNAs (miRNAs) in saliva has expanded field biomarker discovery since these tiny non-coding RNAs affect various processes and diseases. Few reports have linked miRNAs tooth development eruption, none having studied this humans. As a first initiative describe whose modulations may reflect developing erupting teeth, we quantified levels 730 children varying dentition stages: edentulous (newborns), deciduous permanent by megaplex stemloop reverse-transcription quantitative PCR. three groups expressed 193, 181 192 miRNAs, respectively, where 125 had consistent expression. remaining inter-group variations from 5 hundreds fold, most either increasing or decreasing trend going permanent. A literature survey epithelial found were present saliva. Moreover, many expression differences between previously documented functions proliferation, cell cycle, apoptosis other cellular behaviours key dynamics morphogenesis. Lastly, same family, such as let-7 miR-200 families, transcribed hairpin, similar patterns. results presented here should serve salivary miRNA dictionary for future studies well childhood diseases associated composition.