Artemis interacts with the Cul4A-DDB1DDB2ubiquitin E3 ligase and regulates degradation of the CDK inhibitor p27

• DOI: 10.4161/cc.10.23.18227
• Publication Date: 2011-12-22T18:42:17Z
• Authors: Yiyi Yan; Xiaoshan Zhang; Randy J. Legerski
• Keywords: DDB1; Ubiquitin-Protein Ligases; CDK inhibitor

Artemis, a member of the SNM1 gene family, is multifunctional phospho-protein that has been shown to have important roles in V(D)J recombination, DNA double strand break repair, and stress-induced cell-cycle checkpoint regulation. We show here Artemis interacts with Cul4A-DDB1 E3 ubiquitin ligase via direct interaction substrate-specificity receptor DDB2. Furthermore, also CDK inhibitor tumor suppressor p27, substrate ligase, both DDB2 are required for degradation p27 mediated by this complex. regulation cell cycle progression normally proliferating cells response serum deprivation. These findings thus define function as an effector Cullin-based ligase-mediated ubiquitylation, demonstrate novel pathway Cul4A-DDB1DDB2-Artemis regulates G1 phase mammalian cells.