Comparison of efficacy ofSalmonella typhimuriumA1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells
Salmonella typhimurium
0301 basic medicine
Nude
pancreatic cancer
Drug Resistance
Mice, Nude
Antineoplastic Agents
GFP
confocal microscopy
Cell Line
Pancreatic Cancer
Mice
03 medical and health sciences
Rare Diseases
amino acid auxotrophy
fluorescence imaging
Stem Cell Research - Nonembryonic - Human
Cell Line, Tumor
Antineoplastic Combined Chemotherapy Protocols
RFP
Animals
Humans
Cancer
Microscopy
Tumor
Microscopy, Confocal
chemoresistance
Stem Cell Research
Xenograft Model Antitumor Assays
3. Good health
stem cell
Pancreatic Neoplasms
Orphan Drug
selective tumor targeting
Treatment Outcome
Drug Resistance, Neoplasm
Confocal
Neoplastic Stem Cells
Neoplasm
Stem Cell Research - Nonembryonic - Non-Human
Biochemistry and Cell Biology
Digestive Diseases
Developmental Biology
DOI:
10.4161/cc.25872
Publication Date:
2013-08-21T20:40:11Z
AUTHORS (15)
ABSTRACT
The XPA1 human pancreatic cancer cell line is dimorphic, with spindle stem-like cells and round non-stem cells. We report here the in vitro IC 50 values of stem-like and non-stem XPA1 human pancreatic cells cells for: (1) 5-fluorouracil (5-FU), (2) cisplatinum (CDDP), (3) gemcitabine (GEM), and (4) tumor-targeting Salmonella typhimurium A1-R (A1-R). IC 50 values of stem-like XPA1 cells were significantly higher than those of non-stem XPA1 cells for 5-FU (P = 0.007) and CDDP (P = 0.012). In contrast, there was no difference between the efficacy of A1-R on stem-like and non-stem XPA1 cells. In vivo, 5-FU and A1-R significantly reduced the tumor weight of non-stem XPA1 cells (5-FU; P = 0.028; A1-R; P = 0.011). In contrast, only A1-R significantly reduced tumor weight of stem-like XPA1 cells (P = 0.012). The combination A1-R with 5-FU improved the antitumor efficacy compared with 5-FU monotherapy on the stem-like cells (P = 0.004). The results of the present report indicate A1-R is a promising therapy for chemo-resistant pancreatic cancer stem-like cells.
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