Listeria monocytogenesinduces host DNA damage and delays the host cell cycle to promote infection

• DOI: 10.4161/cc.27780
• Publication Date: 2014-02-19T23:00:22Z
• Authors: Elsa Leitão; Ana Catarina Costa; Claudia Brito; Lionel Costa; Rita Pombinho; Didier Cabanes; Sandra Sousa
• Keywords: CDC25A; DNA re-replication; CHEK1

AbstractListeria monocytogenes (Lm) is a human intracellular pathogen widely used to uncover the mechanisms evolved by pathogens establish infection. However, its capacity perturb host cell cycle was never reported. We show that Lm infection affects progression, increasing overall duration but allowing consecutive rounds of division. A complete infectious induces S-phase delay accompanied slower rate DNA synthesis and increased levels strand breaks. Additionally, damage/replication checkpoint responses are triggered in an dose-dependent manner through phosphorylation DNA-PK, H2A.X, CDC25A independently from ATM/ATR. While damage induced exogenously favors dissemination, override pathways limits propose replication disturbed culminates breaks, triggering responses, ensuring propagation.Keywords:: damageDNA-PKbacterial infectionListeria monocytogeneshost cycleCDC25A