Mammalian cells contain two functionally distinct PBAF complexes incorporating different isoforms of PHF10 signature subunit

• DOI: 10.4161/cc.28922
• Publication Date: 2014-05-28T19:04:33Z
• Authors: Alexander V Brech...; Sofia G Georgieva; Nataliya V Soshni...
• Keywords: Transcription

The PBAF subtype of the mammalian chromatin remodeling SWI/SNF complex has wide and diverse functions in transcription regulation development, being both activator repressor. However, a mechanism accounting for such functional diversity remains unclear. Human PHF10/BAF45a subunit plays an important role brain development but not been studied sufficiently. We have shown that PHF10 gene encodes 2 types evolutionarily conserved, ubiquitously expressed isoforms are incorporated into mutually exclusive manner. One isoform contains C-terminal tandem PHD fingers, which other replaced by consensus sequence phosphorylation-dependent SUMO 1 conjugation (PDSM). complexes containing different can bind to promoters same genes produce effects on recruitment Pol II promoter level transcription. In addition, it is only with PHD-containing activates proliferation. Our study demonstrates existence functionally cell. It also provides insight molecular structure human characterizes as essential subunit.