The DCX Superfamily 1: Common and Divergent Roles for Members of the Mouse DCX Superfamily

• DOI: 10.4161/cc.5.9.2715
• Publication Date: 2010-11-30T13:04:49Z
• Authors: Frédéric M. Coquelle; Talia Levy; Sven Bergmann; Sharon Grayer Wolf; Daniela Bar-El; Tamar Sapir; Yehuda Brody; Irit Orr; Naama Barkai; Gregor Eichele; Orly Reiner
• Keywords: Doublecortin; Ras superfamily

AbstractThe doublecortin-like (DCX) domains serve as protein-interaction platforms. DCXtandem appear in the product of X-linked doublecortin gene, inretinitis pigmentosa –1 (RP1), well other gene products. Mutations humanDCX are associated with abnormal neuronal migration, epilepsy, and mentalretardation; mutations RP1 a form inherited blindness, whileDCDC2 has been dyslectic reading disabilities. Motivated by possibleimportance this family, thorough analysis to detect all family members themouse was conducted. The DCX-repeat superfamily is composed elevenparalogs, we cloned DCX from nine different genes. Our studyquestioned which functions attributed domain, conserved among thedifferent members. results suggest that proteins DCX-domain haveconserved unique roles microtubule regulation signal transduction. All thetested stimulated assembly vitro. Proteins tandem repeatsstabilized cytoskeleton transfected cells, while those singlerepeats localized actin-rich subcellular structures, or nucleus. tested interacted components JNK/MAP-kinase pathway, only subsetinteracted Neurabin 2, non-overlapping group demonstrated actin association.The sub-specialization some due confined intracellular localization, andprotein interactions may explain success superfamily.