LINC, a Human Complex That is Related to pRB-Containing Complexes in Invertebrates Regulates the Expression of G2/M Genes

• DOI: 10.4161/cc.6.15.4512
• Publication Date: 2010-08-18T18:44:42Z
• Authors: Fabienne Schmit; Michael Korenjak; Mirijam Mannefeld; Kathrin Schmitt; Claudia Franke; Bjorn von Eyss; Sladjana Gagrica; Frank Hanel; Alexander Brehm; Stefan Gaubatz
• Keywords: G2 Phase; 0301 basic medicine; Tumor Suppressor Proteins; Mitosis; Nuclear Proteins; Nerve Tissue Proteins; Retinoblastoma Protein; Cell Line; E2F Transcription Factors; Protein Subunits; 03 medical and health sciences; Drosophila melanogaster; Gene Expression Regulation; Trans-Activators; Animals; Humans; RNA Interference; Caenorhabditis elegans; Promoter Regions, Genetic; Protein Binding

Here we report the identification of LIN complex (LINC), a human multiprotein that is required for transcriptional activation G2/sub>/M genes. LINC related to recently identified dREAM and DRM complexes Drosophila C.elegans contain homologues mammalian retinoblastoma tumor suppressor protein. The core consists at least five subunits including chromatin-associated LIN-9 RbAp48 proteins. dynamically associates with pocket proteins, E2F B-MYB during cell cycle. In quiescent cells, binds p130 E2F4. During cycle entry, E2F4 dissociate switches p107. Chromatin Immunoprecipitation experiments demonstrate large number E2F-regulated promoters in cells. However, RNAi reveal not repression. S phase, selectively genes whose products are mitosis plays an important role their dependent activation.