Induction of pluripotency in human endothelial cells resets epigenetic profile on genome scale

• DOI: 10.4161/cc.9.5.10869
• Publication Date: 2011-01-06T10:52:34Z
• Authors: Maria A. Lagarkova; Maria V. Shutova; Alexandra N. Bogo...; Ekaterina M. Vassina; Evgeny A. Glazov; Ping Zhang; Albert A. Rizvanov; Ilya V. Chestkov; Sergey L. Kiselev
• Keywords: Reprogramming; Cell potency

Reprogramming of a limited number human cell types has been achieved through ectopic expression four transcription factors to yield induced pluripotent stem (iPS) cells that closely resemble embryonic (ESCs). Here, we determined functional and epigenetic properties iPS generated from umbilical vein endothelial (HUVEC) by conventional method direct reprogramming. Retroviral overexpression resets HUVEC the pluripotency. Human cell-derived (endo-iPS) were similar ESCs in morphology, gene expression, vitro vivo differentiation capacity. Endo-iPS efficiently differentiated into cells. Using genome-wide methylation profiling show promoter elements specific genes methylated following reprogramming whereas pluripotency-related promoters hypomethylated levels observed ESCs. Genome-wide analysis CpG sites located regions over than 14,000 indicated endo-iPS highly ES cells, although differences 46 found. Overall was higher somatic. We also during female exhibited reactivation somatically silenced X chromosome. Our findings demonstrate can be status