Role of lipid rafts in the interaction between hTRPC1, Orai1 and STIM1

• DOI: 10.4161/chan.2.6.7055
• Publication Date: 2010-05-26T13:47:40Z
• Authors: Isaac Jardin; Gines M. Salido; Juan A. Rosado
• Keywords: Orai1; Lipid raft; Thapsigargin

AbstractStore-operated Ca2+ entry (SOCE) is a mechanism regulated by the filling state of intracellular stores that requires participation sensor STIM1, which communicates content to plasma membrane Ca2+-permeable channels. We have recently reported Orai1 mediates communication between STIM1 and channel hTRPC1. This event important confer hTRPC1 store depletion sensitivity, thus supporting functional role STIM1-Orai1-hTRPC1 complex in activation SOCE. Here we explored relevance lipid rafts formation Disturbance raft domains, using methyl-β-cyclodextrin, reduces interaction endogenously expressed both upon attenuates thapsigargin-evoked entry. These findings suggest TRPC1, form heteromultimer associated with domains stores.This article related to: