Differential methylation of STOX1 in human placenta

• DOI: 10.4161/epi.5.8.13084
• Publication Date: 2010-11-29T12:19:36Z
• Authors: Marie van Dijk; Sascha Drewlo; Cees B.M. Oudejans
• Keywords: Genomic Imprinting; CpG site; Trophoblast; Differentially methylated regions

The 10q22 chromosomal region with genomic linkage to pre-eclampsia in Dutch females shows a parent-of-origin effect maternal transmission of the Y153H susceptibility allele STOX1 gene. Although CpG island within promoter no differential methylation, this study describes identification differentially methylated (DMR) intron 1 Methylation coincides expression, where high methylation leads reduced expression. In SGHPL-5 extravillous trophoblast cell line allele-specific expression was observed subset cells. could be detected early placenta samples, these samples did show an increase when they were homozygous for allele. Allele-specific column being paternal origin. We conclude that is paternally imprinted, maternally expressed, DMR identified showing parental-specific specific cell-types, hypothesized occur villous cytotrophoblasts, and proven trophoblasts originating from anchoring villus. other (placental) cells independent parental origin, but regulates genotype directing level methylation.