Physical activity and global genomic DNA methylation in a cancer-free population

• DOI: 10.4161/epi.6.3.14378
• Publication Date: 2011-03-21T15:42:27Z
• Authors: Fang Fang Zhang; Roberto Cardarelli; Joan Carroll; Shun Zhang; Kimberly G. Fulda; Karina Gonzalez; Jamboor K. Vishwa...; Alfredo Morabia; Regina M. Santella
• Keywords: genomic DNA; Bisulfite sequencing; Epigenomics; Pyrosequencing; Illumina Methylation Assay

Changes in DNA methylation may represent an intermediate step between the environment and human diseases. Little is known on whether behavioral risk factors modify gene expression through methylation. To assess associated with different levels of physical activity, we measured global genomic using bisulfite converted real time PCR (MethyLight) for LINE-1 peripheral blood 161 participants aged 45-75 years enrolled North Texas Healthy Heart Study activity accelerometer (Actigraph GT1M Monitor). We found that individuals 26-30 min/day had a significantly higher level compared to those ≤ 10 (β=2.52, 95%CI: 0.70, 4.35) However, association was attenuated became statistically insignificant after multivariate adjustment (β=1.24, 95%CI:-0.93, 3.40). There were some suggestions positive non-Hispanics (β=1.50, -0.08, 3.08) warrants further investigation.