Variation of NimC1 expression in Drosophila stocks and transgenic strains

• DOI: 10.4161/fly.25654
• Publication Date: 2013-10-31T00:32:48Z
• Authors: Viktor Honti; Gyöngyi Cinege; Gábor Csordás; Éva Kurucz; János Zsámboki; Cory J Evans; Utpal Banerjee; István Andó
• Keywords: Gene Expression; Genetically Modified; Animals, Genetically Modified; 03 medical and health sciences; Immunologic; Receptors; Genetics; Animals; Drosophila Proteins; Receptors, Immunologic; plasmatocyte; innate immunity; QR180 Immunology / immunológia; marker; Evolutionary Biology; Phagocytes; 0303 health sciences; phagocytosis receptor; Ecology; Genetic Variation; 3. Good health; Drosophila melanogaster; Drosophila; Biotechnology; Developmental Biology

The NimC1 molecule has been described as a phagocytosis receptor, and is being used marker for professional phagocytes, the plasmatocytes, in Drosophila melanogaster. In studies including tumor-biology, developmental biology, cell mediated immunity, monoclonal antibodies (P1a P1b) to antigen are used. As we observed that these did not react with plasmatocytes of several strains genetic combinations, molecular analysis was performed on structure nimC1 gene. found 2 deletions an insertion within gene, which may result production truncated protein. positivity regained by recombining mutation wild-type allele or using mutant lines under heterozygous conditions. By means procedures recombined stock, can be phagocytic cells majority possible backgrounds.