polyis required for nurse cell chromosome dispersal and oocyte polarity in Drosophila

• DOI: 10.4161/fly.4.2.11954
• Publication Date: 2010-05-25T11:59:22Z
• Authors: Stephen Klusza; Wu-Min Deng
• Keywords: Nurse cell; Cell polarity

AbstractDuring Drosophila oogenesis, nurse cells undergo changes in chromosomal morphology, first from the polytenic form to a transient condensed phase known as five-blob configuration, then into diffuse polytenic-polyploid state for remainder of oogenesis. The mechanism by which nurse-cell chromosome dispersal is regulated remains elusive. Mutations several genes, including heterogeneous ribonucleoprotein genes squid (sqd) and hrb27C, alternative splicing factor gene poly U binding 68kDa (pUf68, also half-pint), germ-line-specific ovarian tumor (otu), that produce defects oocyte polarity, suggesting link between these two processes. Here, we characterize novel named poly, which, when mutated germ line, disrupts dispersal, well localization anteroposterior dorsoventral determinants oocyte. We show interacts genetically with hrb27C otu. conclude required polarization germ-line. In addition, our interaction data suggest probably member characterized mRNP complex mediates both