Amelioration of DSS-induced murine colitis by VSL#3 supplementation is primarily associated with changes in ileal microbiota composition.

• DOI: 10.4161/gmic.32147
• Publication Date: 2014-08-04T21:49:10Z
• Authors: Jordan S. Mar; Nabeetha A. Nagal...; Yuanlin Song; Michio Onizawa; Jae Woo Lee; Susan V Lynch
• Keywords: Dysbiosis; Cecum

Inflammatory bowel diseases encompass gastrointestinal illnesses typified by chronic inflammation, loss of epithelial integrity and microbiota dysbiosis. In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in murine model Dextran Sodium Sulfate induced colitis examine both their efficacy ameliorating disease impact on niche-specific microbial communities the lower GI tract. Colitis was C57BL/6 mice administering 3% DSS drinking water for 10 days prior one three treatment plans: daily (VSL#3) supplementation 3 days, single tail vein injection 1x106 mesenchymal cells, or both. Ileal, cecal colonic sections were collected histological analyses. Microbiota profiling revealed distinct bacterial community compositions ileum, cecum colon control untreated animals, all which predicted silico be enriched number discrete KEGG pathways, indicating compositional functional niche specificity healthy animals. DSS-treatment perturbed composition niches with ileal exhibiting greatest change relative Each group exhibited treatment-specific alterations tract, though scores only improved VSL#3-treated The most profoundly altered this animals characterized significant Enterobacteriaceae enrichment compared colitic (P < 0.05).