Evaluation of the overall IFN-γ and IL-17 pro-inflammatory responses after DNA therapy of tuberculosis

• DOI: 10.4161/hv.23417
• Publication Date: 2013-01-30T23:21:27Z
• Authors: Carlos R. Zárate-...; Rodrigo F. Rodrigues; Patricia R. M. Souza; Wendy M. Rios; Luana S. Soares; Rogério S. Rosada; Izaíra T. Brandão; Ana Paula Masson; Elaine M. Floriano; Simone G. Ramos; Celio L. Silva
• Keywords: CD4-Positive T-Lymphocytes; Inflammation; Mice, Inbred BALB C; Interleukin-17; Receptors, Antigen, T-Cell, gamma-delta; Genetic Therapy; CD8-Positive T-Lymphocytes; 3. Good health; Disease Models, Animal; Interferon-gamma; Mice; 03 medical and health sciences; 0302 clinical medicine; Animals; Tuberculosis; Female; Lung

Despite the enormous efforts displayed globally in fight against tuberculosis, disease incidence has modified slightly, which led to a renewed interest immunotherapy. In general, successful immunotherapeutic candidates tuberculosis are agents that can trigger strong, specific pro-inflammatory responses, especially of T-helper (Th) 1 pattern. However, how these effectively kill bacteria without eliciting immunopathology is not well understood. We reasoned that, addition immune response elicited by immunotherapy, evaluation overall responses should provide additional and valuable information will be useful avoiding immunopathology. evaluated IFN-γ IL-17 among CD4(+), CD8(+) γδ T cells lungs mice were infected with M. treated DNA vaccine an regimen. Our results demonstrate combat pathogen develop Th1 therapeutic antigen have reduced lung inflammation, present parallel fine-tuning total IFN-γ- IL-17-mediated immunity lungs. This modulation involves reducing Th17 cell population, augmenting produce increasing frequency. These stress importance broad only at time evaluate new interventional strategies but also non-conventional cells, such as lymphocytes.