Comparative study of the immunogenicity in mice and monkeys of an inactivated CA16 vaccine made from a human diploid cell line

• DOI: 10.4161/hv.28083
• Publication Date: 2014-02-28T21:50:27Z
• Authors: Erxia Yang; Chen Cheng; Ying Zhang; Jingjing Wang; Yanchun Che; Jing Pu; Chenghong Dong; Longding Liu; Zhanlong He; Shuaiyao Lu; Yuan Zhao; Li Jiang; Yun Liao; Congwen Shao; Qihan Li
• Keywords: ELISPOT; Enterovirus 71; Vero cell; Inactivated vaccine

The coxsackie A16 virus (CA16), along with enterovirus 71 (EV71), is a primary pathogen that causes hand, foot, and mouth disease (HFMD). To control HFMD, CA16, EV71 vaccines are needed. In this study, an experimental inactivated CA16 vaccine was prepared using human diploid cells, the vaccine's immunogenicity analyzed in mice rhesus monkeys. results showed neutralizing antibody developed dose-dependent manner, sustained for 70 days average GMT (geometric mean titer) level of 80 to 90 immunized mouse 56 higher than 300 had cross-neutralizing activity against different viral strains (genotype A B), specific IFN-γ-secreting cell response activated by these ELISPOT assay. This study provides evidence potential use as candidate vaccines.