Metabolomic analysis of pancreatic β-cell insulin release in response to glucose

• DOI: 10.4161/isl.20141
• Publication Date: 2012-07-23T19:42:26Z
• Authors: Mei Huang; Jamie W. Joseph
• Keywords: Metabolic pathway; Metabolome

Defining the key metabolic pathways that are important for fuel-regulated insulin secretion is critical to providing a complete picture of how nutrients regulate secretion. We have performed detailed metabolomics study clonal β-cell line 832/13 using gas chromatography-mass spectrometer (GC-MS) investigate potential coupling factors link Mid-polar and polar metabolites, extracted from β-cells, were derivatized then run on GC/MS identify quantify metabolite concentrations. Three hundred fifty-five out 527 chromatographic peaks could be identified as metabolites by our metabolomic platform. These allowed us perform systematic analysis involved in glucose-stimulated (GSIS). Of these 41 consistently biomarker GSIS orthogonal partial least-squares (OPLS). Most common including glycolytic, sorbitol-aldose reductase pathway, pentose phosphate TCA cycle suggesting play an role GSIS. Lipids related products also shown contribute clustering high glucose sample groups. Amino acids lysine, tyrosine, alanine serine upregulated whereas aspartic acid was downregulated amino might In summary, coordinated signaling cascade elicited metabolism pancreatic β-cells revealed platform new conceptual framework future research and/or drug discovery.