Homogenous photocatalytic decontamination of prion infected stainless steel and titanium surfaces

• DOI: 10.4161/pri.27180
• Publication Date: 2013-11-19T03:28:25Z
• Authors: Chrysanthi Berber...; Konstantinos Xant...; Ioannis Paspaltsis; Athanasios Lourbo...; Eleni Polyzoidou; Theodoros Sklaviadis; Ioannis Poulios
• Keywords: Human decontamination

Prions are notorious for their extraordinary resistance to traditional methods of decontamination, rendering transmission a public health risk. Iatrogenic Creutzfeldt-Jakob disease (iCJD) via contaminated surgical instruments and medical devices has been verified both experimentally clinically. Standard prion inactivation by sodium hydroxide or hypochlorite have failed, in some cases, fully remove infectivity, while they often impractical routine applications. Prion accumulation peripheral tissues indications human-to-human bloodborne transmission, highlight the need novel, efficient, yet user-friendly inactivation. Here we show vitro vivo that homogenous photocatalytic oxidation, mediated photo-Fenton reagent, potential inactivate pathological isoform adsorbed on metal substrates. Photocatalytic oxidation with 224 μg mL(-1) Fe (3+), 500 h(-1) H 2O 2, UV-A 480 min lead 100% survival golden Syrian hamsters after intracranial implantation stainless steel wires infected 263K strain. Interestingly, treatment titanium wires, under same experimental conditions, prolonged interval significantly, but failed eliminate result correlate increased adsorption PrP(Sc) titanium, comparison steel. Our findings strongly indicate our, user--and environmentally--friendly protocol can be safely applied decontamination surfaces.