Real-time quaking-induced conversion

• DOI: 10.4161/pri.5.3.16893
• Publication Date: 2011-12-01T23:44:04Z
• Authors: Ryuichiro Atarashi; Kazunori Sano; Katsuya Satoh; Noriyuki Nishida
• Keywords: Chronic wasting disease; Bovine spongiform encephalopathy; Transmissible spongiform encephalopathy

We recently developed a new in vitro amplification technology, designated “real-time quaking-induced conversion (RT-QUIC)”, for detection of the abnormal form prion protein (PrPSc) easily accessible specimens such as cerebrospinal fluid (CSF). After assessment more than 200 CSF from Japanese and Australian patients, we found no instance false positive, 80% accuracy correct diagnosis sporadic Creutzfeldt–Jakob disease (sCJD). Furthermore, RT-QUIC can be applied to other diseases, including scrapie, chronic wasting (CWD), bovine spongiform encephalopathy (BSE), is able quantify seeding activity when combined with an end-point dilution samples. These results indicate that RT-QUIC, its high sensitivity specificity, will great use early, rapid specific assay diseases.