Overexpression ofRab16Agene in indica rice variety for generating enhanced salt tolerance

• DOI: 10.4161/psb.19646
• Publication Date: 2012-06-27T09:55:26Z
• Authors: Moumita Ganguly; Karabi Datta; Aryadeep Roychoud...; Dipak Gayen; Dibyendu N. Sengupta; Swapan K. Datta
• Keywords: Genetically modified rice

We report here the overexpression of Rab16A full length gene (promoter + ORF), from salt-tolerant indica rice Pokkali, in salt-susceptible variety Khitish, via particle bombardment. Molecular analysis transgenics revealed stable integration transgene upto T2 generation. High level expression (driven by its own stress-inducible promoter), as well protein, was detectable leaves under simulated salinity stress (250 mM NaCl, 24 h); being higher than wild type (WT) plants. The transcript also increased gradually with seed maturity, maximal accumulation at 30 d after pollination (DAP) i.e., fully matured seeds, explaining protective role during maturation. Enhanced tolerance to observed plants transformed Rab16A. superior physiological performances salt treatment were reflected lesser shoot or root inhibition, reduced chlorophyll damages, Na+ and loss K+, proline content compared WT All these results indicated that overproduction RAB16A protein enable them display enhanced improved traits. Our work demonstrates profound potential Group 2 LEA proteins (to which belongs to) conferring crop through their genetic manipulation.