Arabidopsisscaffold protein RACK1A interacts with diverse environmental stress and photosynthesis related proteins

• DOI: 10.4161/psb.24012
• Publication Date: 2013-04-09T15:50:24Z
• Authors: Nabanita Kundu; Uvetta Dozier; Laurent Deslandes; Imre E. Somssich; Hemayet Ullah
• Keywords: 14-3-3 protein; Ribosomal protein

Scaffold proteins are known to regulate important cellular processes by interacting with multiple modulate molecular responses. RACK1 (Receptor for Activated C Kinase 1) is a WD-40 type scaffold protein, conserved in eukaryotes, from Chlamydymonas plants and humans, expresses ubiquitously plays regulatory roles diverse signal transduction stress response pathways. Here we present the use of Arabidopsis RACK1A, predominant isoform 3-member family, as bait screen split-ubiquitin based cDNA library. In total 97 dehydration, salt stress, ribosomal photosynthesis pathways found potentially interact RACK1A. False positive interactions were eliminated following extensive selection growth potentials. Confirmation sub-set selected demonstrated through co-transformation individual plasmid containing respective bait. Interaction points role RACK1A cross-talk between signaling Promoter analysis photosynthetic pathway genes revealed transcription factor binding sites. be multifunctional protein current identification potential future vivo elucidations physiological basis such will shed light on possible mechanisms that uses