MicroFilament Analyzer identifies actin network organizations in epidermal cells ofArabidopsis thalianaroots

• DOI: 10.4161/psb.24821
• Publication Date: 2013-06-26T18:29:43Z
• Authors: Eveline Jacques; Michal Lewandowski; Jan Buytaert; Yves Fierens; Jean-Pierre Verbelen; Kris Vissenberg
• Keywords: Epidermis (zoology); Plant cell

The plant cytoskeleton plays a crucial role in the cells' growth and development during different developmental stages it undergoes many rearrangements. In order to describe arrangements of F-actin root epidermal cells Arabidopsis thaliana, recently developed software MicroFilament Analyzer (MFA) was exploited. This enables high-throughput identification quantification orientation filamentous structures on digital images highly standardized fast way. Using confocal microscopy transgenic GFP-FABD2-GFP plants actin visualized epidermis. MFA analysis revealed that early cell is organized mainly random pattern. As grow, they preferentially adopt longitudinal organization, pattern also preserved largest cells. evolution from young old cells, an approximately even distribution transverse, oblique or combined orientations always present besides switch oriented cytoskeleton.