Arabidopsis GH3.5 regulates salicylic acid-dependent and both NPR1-dependent and independent defense responses

• DOI: 10.4161/psb.3.8.5748
• Publication Date: 2010-10-28T20:08:24Z
• Authors: Zhongqin Zhang; Muyang Wang; Zhimiao Li; Qun Li; Zuhua He
• Keywords: Pseudomonas syringae; NPR1

The cross-talk between plant disease resistance and development is fundamental to understanding systemic physiological processes during pathogen attack. Our previous study showed that the Arabidopsis GH3.5 gene acts as a bifunctional modulator of salicylic acid (SA)-mediated auxin-mediated susceptibility Arabidopsis-Pseudomonas syringae interaction well development. Here, we further role mechanism involved in SA-dependent defense pathway. Transcript histochemical analysis promoter::GUS reporter expression indicate expressed with strong temporal spatial manner predominant divisional tissues. Upon bacterial challenge, GUS activity induced junction tissue around infiltrated zone higher levels vasculature pattern different incompatible compatible interactions. Exogenous SA application enhances activation-tagged mutant gh3.5-1D, while GH3.5-mediated enhancement depleted deficient gh3.5-1D/NahG double mutant, indicating modulates response through Furthermore, growth gh3.5-1D/npr1 treated indicates SA-mediated both NPR1-dependent independent pathways.