Contributions of transcription and mRNA decay to gene expression dynamics of fission yeast in response to oxidative stress

• DOI: 10.4161/rna.29196
• Publication Date: 2014-07-09T16:22:05Z
• Authors: Samuel Marguerat; Katherine Lawler; Alvis Brazma; Jürg Bähler
• Keywords: ribosome biogenesis; Transcription; Schizosaccharomyces

The cooperation of transcriptional and post-transcriptional levels control to shape gene regulation is only partially understood. Here we show that a combination two simple non-invasive genomic techniques, coupled with kinetic mathematical modeling, affords insight into the intricate dynamics RNA in response oxidative stress fission yeast Schizosaccharomyces pombe. This study reveals dominant role stress, but also points first minutes after induction as critical time when coordinated mRNA turnover can support transcription for rapid regulation. In addition, uncover specialized expression strategies associated distinct functional groups, such simultaneous repression destabilization genes encoding ribosomal proteins, delayed varying contribution ribosome biogenesis genes, roles stabilization functioning protein degradation, adjustment both during adaptation stress. We regulated independently bZIP factor Atf1p are predominantly controlled by turnover, identify putative cis-regulatory sequences different response. highlights multi-faceted interplay between dynamic regulatory