Characterization of a carcinogenesis-associated long non-coding RNA

• DOI: 10.4161/rna.9.1.18332
• Publication Date: 2012-02-01T16:40:43Z
• Authors: Feng Yang; Fan Yi; Zhiguo Zheng; Zhiqiang Ling; Jianv Ding; Jiangfeng Guo; Weimin Mao; Xiongbiao Wang; Xiaoxia Wang; Xianfeng Ding; Zicai Liang; Quan Du
• Keywords: 0301 basic medicine; RNA, Untranslated; Genome, Human; Gene Expression Profiling; Cell Cycle; Genetic Vectors; Transfection; 3. Good health; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; HEK293 Cells; Chromosomes, Human, Pair 1; Case-Control Studies; Neoplasms; Biomarkers, Tumor; Humans; RNA, Messenger; RNA, Neoplasm; Cloning, Molecular; HeLa Cells

A negative selection strategy was used in the present study to isolate long polyA-minus RNAs from the total transcriptome and a long non-coding RNA named Yiya was identified. Yiya is a 1.9 kb long intergenic ncRNA gene mapped to chromosome 1q41, a well-established cancer susceptibility locus. Expression profiling revealed a general and regulated expression pattern of Yiya in major tissues, and more interestingly, identified elevated mRNA levels in different cancers. Quantitative analysis further demonstrated a dynamic regulation of Yiya expression in cell cycle progression, suggesting that it was involved in cell cycle regulation. Supporting this, overexpression of Yiya promotes cell cycle progression at the G1/S transition, therefore identifying Yiya as a cell-cycle-associated long non-coding RNA.