GTP-dependent scaffold formation in the GTPase of Immunity-Associated Protein family

• DOI: 10.4161/sgtp.2.1.14938
• Publication Date: 2011-04-05T17:47:15Z
• Authors: David Schwefel; Oliver Daumke
• Keywords: GTP-binding protein regulators; Protein family

GTP ases of Immunity-Associated Proteins (GIMAPs) are a family guanine nucleotide binding (G) proteins which implicated in the regulation apoptosis lymphocytes. GIMAPs composed an amino-terminal G domain and carboxy-terminal extensions varying size. Our recent biochemical structural analysis representative GIMAP member, GIMAP2, revealed molecular basis GTP-dependent oligomerization involves two interfaces domain. Whereas amphipathic helix α7 C-terminal extension closely folds against GDP-bound state, it might be released GTP-bound state to assemble interaction partners. We also showed that GIMAP2 oligomer functions at surface lipid droplets Jurkat T cell line. Here, we review our work discuss as protein scaffold controlling apoptosis.