Anti-fatigue effect of tormentic acid through alleviating oxidative stress and energy metabolism-modulating property in C2C12 cells and animal models

• DOI: 10.4162/nrp.2023.17.4.670
• Publication Date: 2023-07-26T00:27:35Z
• Authors: Ho-Geun Kang; Jin-Ho Lim; Hee-Yun Kim; Hyunyong Kim; Hyung-Min Kim; Hyun-Ja Jeong
• Keywords: Aspartate transaminase; Malondialdehyde; Creatine kinase

Oxidative stress is caused by reactive oxygen species and free radicals that accelerate inflammatory responses exacerbate fatigue. Tormentic acid (TA) has antioxidant anti-inflammatory properties. Thus, the aim of present study to determine fatigue-regulatory effects TA in H2O2-stimulated myoblast cell line, C2C12 cells treadmill test (TST) forced swimming (FST) animal models.In vitro study, were pretreated with before stimulation H2O2. Then, malondialdehyde (MDA), lactate dehydrogenase (LDH), creatine kinase (CK) activity, tumor necrosis factor (TNF)-α, interleukin (IL)-6, superoxide dismutase (SOD), catalase (CAT), glycogen, viability analyzed. In vivo ICR male mice administered or distilled water orally daily for 28 days. FST TST then performed on last day. addition, biochemical analysis serum, muscle, liver was performed.TA dose-dependently alleviated levels MDA, LDH, CK TNF-α, IL-6 without affecting cytotoxicity. increased SOD CAT activities glycogen cells. models, decreased immobility time significantly while increasing exhaustion weight fluctuations. The studies showed SOD, CAT, citrate synthase, fatty administration, whereas reduced glucose, lactate, CK, cytokines, alanine transaminase, aspartate blood urea nitrogen, cortisol compared control group.TA improves fatigue modulating oxidative energy metabolism models. Therefore, we suggest can be a powerful substance healthy functional foods therapeutics improve