Investigating the Expression and Function of HIF-1α in Neocaridina davidi During Embryo Cleavage Stage

• DOI: 10.4194/trjfas25726
• Publication Date: 2024-12-09T05:07:15Z
• Authors: Ran Li; Jiale Yang; Runlin Zhou; Ran Lin; Jinsheng Sun
• Keywords: Cleavage (geology)

Neocaridina davidi, a member of Decapoda within Crustacea, stands out due to its petite stature, robust reproductive capabilities and short molting cycle. Consequently, it has emerged as valuable experimental model for investigations spanning ecology, development, physiology, toxicology. Despite the pivotal role Hypoxia Inducible Factor-1α (HIF-1α) in diverse physiological processes like biological hypoxia stress, cellular growth, stress resistance, functionality crustaceans remains underexplored. Moreover, function HIF-1α crustaceans, especially embryonic stages, have been insufficiently addressed. This study delves into during development. Initially, complete sequence gene was acquired. Notably, expression cleavage stage surpassed that subsequent phases. Subsequently, dsRNAHIF-1α introduced sexually mature shrimp, revealing inhibitory impact dsRNA on parental generation could be inherited by offspring, exerting specific silencing effect embryo. The consequences embryos manifested varying degrees premature division termination, besides, glycolysis also affected suppression. These results provide data support understanding early development functions it.