Effect of Hypoxia on the Expression of a Subset of Proliferation Related Genes in IRE1 Knockdown U87 Glioma Cells

• DOI: 10.4236/abc.2017.76014
• Publication Date: 2018-03-15T09:16:54Z
• Authors: Dariia O. Tsymbal; Dmytro O. Minchenko; Oksana S. Hnatiuk; Olha Y. Luzina; Oleksandr H. Minc...
• Keywords: Clusterin; ATF6

We have studied the expression of a subset genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well their hypoxic regulation. It was shown that levels activating transcription factor 6 (ATF6), clusterin (CLU), adhesion G protein-coupled receptor E5 (ADGRE5), transglutaminase 2, C polypeptide (TGM2), leukemia inhibitory (LIF), phosphoserine aminotransferase 1 (PSAT1), glyoxalase I (GLO1) and tetraspanin 13 (TSPAN13) are significantly down-regulated signaling enzyme. also subjected to hypoxia, PSAT1, TSPAN13, EIF2AK3, TGM2 were up-regulated, whereas ATF6 gene down-regulated. At same time, LIF, CLU, ADGRE5 did not change response treatment. Furthermore, inhibition IRE1, key effector an unfolded protein pathway, modified effect hypoxia on most genes. Present study demonstrates regulation these changes possibly contributed suppression without enzyme function.