Expression of Difficult-to-Express Proteins, Human IL-12 and IFN-<i>γ</i>

• DOI: 10.4236/ajmb.2021.112003
• Publication Date: 2021-03-03T05:57:23Z
• Authors: Yoshihito Hosaka; Shun Matsutani; Shinya Kawate; Kei Itoh; Atsuko Miura; Yukaze Mizoura; Sayumi Yamada; Seiya Uemura; Hiroshi Konno; Ewa Grave; Hideki Wakui; Hideaki Itoh
• Keywords: 0303 health sciences; 03 medical and health sciences; 3. Good health

It is known to be that lactic acid bacteria induce the IL-12. IL-12 activates NK cells and promotes production of IFN-γ. IFN-γ macrophages, resulting in enhanced phagocytosis bactericidal activity. We have been investigating fermented foods activate immune function. For purpose, a specific antibody required. tried express IL-12p35 by usual method, but was not expressed at all. In present study, we constructed, purified human obtained against IL-12p35. also established method for expressing poorly proteins. The can applied purification proteins production.