Human Milk Oligosaccharides Enhance Innate Immunity to Respiratory Syncytial Virus and Influenza <i>in Vitro</i>

• DOI: 10.4236/fns.2014.514151
• Publication Date: 2014-08-15T02:20:49Z
• Authors: Geralyn Duska-McEwen; Albert P. Senft; Teah L. Ruetschil...; Edward G. Barrett; Rachael H. Buck
• Keywords: 0301 basic medicine; 03 medical and health sciences; 3. Good health

Human milk oligosaccharides (HMO) contribute to innate immunity by enhancing growth of beneficial bacteria, epithelial cell maturation and mucosal barrier integrity. They have immunomodulatory effects can block pathogen binding host surface glycans or receptors. We investigated the 2’-fucosyllactose (2’FL), 6’-sialyllactose (6’SL), 3’-sialyllactose (3’SL) lacto-N-neoTetraose (LNnT) on human respiratory lines peripheral blood mononuclear cells (PBMCs) following viral infectionin vitro. Expression cytokines load were monitored in infected cells. These biomarkers selected since cytokine levels (IP-10, MIP-1α, IL-6, IL-8, TNF-α) been correlated with disease severity syncytial virus (RSV) influenza (IAV) vivo. 2’FL significantly decreased RSV associated (IL-6, MIP-1α) inflammation (TNF-α, MCP-1) airway LNnT 6’SL IAV dose-dependently down-regulated IP-10 TNF-α PBMCs. HMO at below found breast enhance viruses vitro may interact directly modulate immunity.