Effect of the Jianpi Bushen Prescription on expressions of the Wnt3a and Cyclin D1 genes in radiation-damaged mice

• DOI: 10.4238/2013.october.3.1
• Publication Date: 2013-10-07T14:31:01Z
• Authors: D.C. He; J.J. Xiao; Y. Zhang; H. Lin; X.J. Ding; Y. Tu
• Keywords: Male; Down-Regulation; Gene Expression; Bone Marrow Cells; Radiation-Protective Agents; 3. Good health; Mice; Radiation Injuries, Experimental; 03 medical and health sciences; 0302 clinical medicine; Granulocyte-Macrophage Progenitor Cells; Wnt3A Protein; Animals; Cyclin D1; Medicine, Chinese Traditional; Cells, Cultured; Whole-Body Irradiation; Signal Transduction

The effects of the traditional Chinese drug Jianpi Bushen Prescription (JBP) were investigated on expressions Wnt3a and Cyclin D1 genes in radiation-damaged mice. radiation damage model was induced Kumming mice by single total body irradiation treatment for 9 days. Mice divided into group, low-dose (100%) JBP high-dose (200%) or batyl alcohol group (positive control), which administered twice a day mRNA protein detected bone marrow mononuclear cells real-time polymerase chain reaction Western blot, whereas situ hybridization. significantly downregulated compared with all other groups (P < 0.05). positive cell rate expression number granulocyte macrophage colonies decreased relative to Furthermore, Wnt3a, cells, higher than In summary, plays protective role radiation-induced through activation signaling pathway, promotes transcription D1.