Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep

• DOI: 10.4238/2014.april.8.7
• Publication Date: 2014-04-10T17:18:06Z
• Authors: M. Yang; Y.J. Yue; T.T. Guo; J.L. Han; J.B. Liu; J. Guo; X.P. Sun; R.L. Feng; Y.Y. Wu; C.F. Wang; L.P. Wang; B.H. Yang
• Keywords: High Resolution Melt; Melting curve analysis; STR analysis

Variation in microsatellite or simple sequence repeat (SSR) loci has, until recently, relied heavily on the use of gel-based methods that can be both time consuming and difficult to genotype.Non systems are therefore important increase simplicity improve turn-around without compromising assay sensitivity accuracy.In this report, we assessed latest non-gelbased methods, high-resolution melting (HRM) curve analysis.HRM is a technique monitors exactly decreasing fluorescence intercalating dye process dissociation double-stranded DNA.The measurement immediately follows polymerase chain reaction one-step, closed-tube method.Four SSR different complexity sheep, namely MAF209, MCM140, CB226, SRCRSP5, were using LightScanners System with LC Greens PLUS DNA binding dye.In order accuracy genotyping, ©FUNPEC-RP www.funpecrp.com.