Effect of hyperphosphatemia on gene expression of the Na-Pi cotransporter in rats

• DOI: 10.4238/2015.december.30.1
• Publication Date: 2016-01-04T16:27:21Z
• Authors: C. Zhang; L.-M. Shi; Y. Li; Q.-G Zhu; C.-L. Jin; H.-P. Wang; L. Zhou; X.-P. Hu; L. Zhang; H. Wang; G.-J. Yuan; L. Xu; Y.-K. Zhao; Y. Chou
• Keywords: Sodium-Phosphate Cotransporter Proteins, Type III; Kidney; Sodium-Phosphate Cotransporter Proteins, Type IIa; Phosphates; Rats; 3. Good health; Hyperphosphatemia; Rats, Sprague-Dawley; 03 medical and health sciences; 0302 clinical medicine; Gene Expression Regulation; Parathyroid Hormone; Animals; Calcium; RNA, Messenger

We investigated the effect of high phosphorus content on sodium-phosphate cotransporter (NaPi-IIa and NaPi-IIl).Forty-eight Sprague-Dawley rats were divided into 3 groups: high-phosphorus group (HP) with fructose diphosphate sodium injection; self-manufactured lowphosphorus diet (LP); normal (NP).At 1st, 2nd, 4th, 6th weeks, 4 from each sacrificed for detecting serum levels calcium, phosphorus, intact parathyroid hormone.Semi-quantitative retrovirus-polymerase chain reaction was used to detect expression NaPi-IIa NaPi-III mRNA in kidney.At hormone HP significantly higher than those LP NP groups (P < 0.05).Serum calcium showed no difference > 0.05).Comparing groups, reduced 0.05), while began NaPi ©FUNPEC-RP www.funpecrp.com.brGenetics Molecular Research 14 (4): 19404-19410 (2015) increasing at 4th week 0.05).At HP, LP, clear differences group, slightly increased compared 0.05).Hyperphosphatemia affected expression, a factor promote an increase independently calcium.