DNA barcoding for efficient identification of Ixiolirion species (Ixioliriaceae)

• DOI: 10.4238/2015.march.13.19
• Publication Date: 2015-03-13T19:36:31Z
• Authors: Y. Zhao; Y. Li; Y. Liu; Y.F. Yang
• Keywords: DNA Barcoding; Identification; Species Identification

Ixiolirion is a genus of unresolved taxonomy.DNA barcoding technique that allows species identification using standardized DNA sequences.In this study, total 23 individuals, representing 2 Chinese species, were sampled to test the effectiveness 3 barcodes [internal transcribed spacer (ITS), chloroplast tRNA intron, and megakaryocyte-associated tyrosine kinase] for identification.Of barcodes, ITS displayed maximum level polymerase chain reaction sequencing success as well highest sequence variation.Intra-specific distances ITS, kinase 0, 0-0.1%, respectively, with 8.3, 0.6, 0.5% mean inter-specific distances, respectively.All individuals each formed monophyletic group (clade) in neighborjoining trees constructed single-DNA barcodes.Our results demonstrated megakaryocyteassociated markers could be used efficiently ©FUNPEC-RP www.funpecrp.com.br