LEF1 influences diabetic retinopathy and retinal pigment epithelial cell ferroptosis via the miR-495-3p/GRP78 axis through lnc-MGC

• DOI: 10.4239/wjd.v16.i3.92003
• Publication Date: 2025-01-20T20:02:58Z
• Authors: Yi-Yi Luo; Xue-Ying Ba; Ling Wang; Ye-Pin Zhang; Hong Xu; Pei-Qi Chen; Li-Bo Zhang; Jian Han; Heng Luo
• Keywords: Viability assay

BACKGROUND Diabetic retinopathy (DR) is one of the major eye diseases contributing to blindness worldwide. Endoplasmic reticulum (ER) stress in retinal cells a key factor leading inflammation and vascular leakage DR, but its mechanism still unclear. AIM To investigate potential LEF1 related RNAs DR. METHODS ARPE-19 were exposed high levels glucose for 24 hours simulate diabetic environment. Intraperitoneally injected streptozotocin was used induce rat model The expression genes proteins measured by RT-qPCR Western blotting; lnc-MGC miR-495-3p detected fluorescent situ hybridization; CCK-8 TUNEL assays detect cell viability apoptosis; enzyme-linked immunosorbent assay inflammatory factors; dual-luciferase gene verify targeting relationship; retina observed HE staining. RESULTS have binding sites, can regulate /GRP78 molecular axis. In glucose-treated cells, aggravated, intracellular reactive oxygen species concentration increased, reduced, apoptosis ER response intensified, ferroptosis increased. As an chaperone, GRP78 regulates under , whereas inhibiting further downregulate increase level sequentially alleviate occurrence development Animal experiments indicated that knockdown affect /miR-495-3p signaling axis restrain progression CONCLUSION through which affects restrains DR pigment epithelial cells.