Anti-HIV activity and mechanism of Cynanchum otophyllum glucan sulfate in vitro

• DOI: 10.4268/cjcmm20111821
• Publication Date: 2011-12-03T08:48:31Z
• Authors: TAO Jian
• Keywords: 0301 basic medicine; Cynanchum; Anti-HIV Agents; Plant Extracts; Plant Roots; Cell Line; 3. Good health; Cell Fusion; Inhibitory Concentration 50; 03 medical and health sciences; Viral Envelope Proteins; HIV-1; Humans; Glucans; Cell Proliferation

To study anti-HIV activity and mechanism of Cynanchum otophyllum glucan sulfate in vitro.Anti-HIV-1 activity was detected with syncytial formation assay and quantitative P24 enzyme-linked immunosorbent assay (ELISA); cytotoxicity was tested with MTT colorimetric assay. Antiviral mechanism was investigated by fusion inhibition, time of addition and pre-treatment experiments.The 50% inhibition concentrations (IC50) of PS20 for HIV-1(IIIB), HIV-1(Ada-M), and HIV-1(Bal), were 0.26, 0.46, 0.90 micromol x L(-1), respectively. Studies on antiviral mechanism of PS20 showed that target molecule may be viral envelope protein.The results suggested that PS20 had high anti-HIV activity and was worth to be studied further.