Diagnostic Accuracy of Kato–Katz, FLOTAC, Baermann, and PCR Methods for the Detection of Light-Intensity Hookworm and Strongyloides stercoralis Infections in Tanzania

• DOI: 10.4269/ajtmh.13-0268
• Publication Date: 2014-01-21T03:35:52Z
• Authors: Stefanie Knopp; Nahya Salim; Tobias Schindler; Dimitrios A. Kara...; Julian Rothen; Omar Lweno; Alisa S. Mohammed; Raymond Singo; Myrna Benninghoff; Anthony A. Nsojo; Blaise Genton; Claudia Daubenberger
• Keywords: Strongyloides stercoralis; Hookworm Infections; Strongyloidiasis; Hookworm Infections

Sensitive diagnostic tools are crucial for an accurate assessment of helminth infections in low-endemicity areas. We examined stool samples from Tanzanian individuals and compared the accuracy a real-time polymerase chain reaction (PCR) with FLOTAC technique Kato–Katz method hookworm Baermann Strongyloides stercoralis detection. Only had higher sensitivity than diagnosis; sensitivities PCR were equal. very low S. The cycle threshold values negatively correlated logarithm egg larvae counts. median count was significantly lower false negatives true positives. All methods failed to detect low-intensity infections. New approaches needed monitoring progressing control programs, confirmation elimination, or surveillance disease recrudescence.