Infectivity of the Subspecies of the Leishmania braziliensis Complex in Vivo and in Vitro

• DOI: 10.4269/ajtmh.1990.43.623
• Publication Date: 2017-05-10T16:43:55Z
• Authors: J. A. Rey; B. L. Travi; A. Z. Valencia; N. G. Saravia
• Keywords: Infectivity; Leishmania braziliensis; Subspecies

The infectivity of Leishmania braziliensis ssp. in relation to their growth kinetics Senekjie's medium was determined using the human macrophage cell line U937 and inbred hamsters. In both systems, shown be distinctive for each subspecies. While L. b. panamensis promastigotes from 6-day-old cultures (early stationary phase) were more infective than parasites any other culture day, guyanensis reached maximum on days 8–10 day 10 (late phase growth), respectively. Although occurred during growth, strict dependency not observed. populations these composed mostly small, highly motile with flagella 2–3 times length bodies. These resembled forms transmitted by sand fly vector. A distinct pathological picture characterized disease caused different WHO reference strains subspecies hamsters: developed most severe lesions, while moderate inconspicuous lesions observed when braziliensis, respectively, constituted inocula.