CONGENITAL CHAGAS DISEASE IN BOLIVIA IS NOT ASSOCIATED WITH DNA POLYMORPHISM OF TRYPANOSOMA CRUZI

• DOI: 10.4269/ajtmh.2006.75.871
• Publication Date: 2018-09-01T06:22:34Z
• Authors: MYRNA VIRREIRA; CRISTINA ALONSO-VEGA; MARCO SOLANO; JUAN JIJENA; LAURENT BRUTUS; ZULEMA BUSTAMANTE; CARINE TRUYENS; DOMINIQUE SCHNEIDER; FAUSTINO TORRICO; YVES CARLIER; MICHAL SVOBODA
• Keywords: Bolivia; TRANSMISSION MERE ENFANT; Infectious Disease Transmission; Trypanosoma cruzi; Chagas Disease -- epidemiology; Trypanosoma cruzi -- classification; Protozoan -- analysis; 03 medical and health sciences; DIAGNOSTIC; 0302 clinical medicine; Genetic; TEST ELISA; INFECTION; TECHNIQUE PCR; Vertical; Animals; Humans; Chagas Disease; MARQUEUR MOLECULAIRE; Polymorphism; Trypanosoma cruzi -- isolation & purification; PARASITE; MUTATION; RECOMBINAISON; Polymorphism, Genetic; Chagas Disease -- parasitology; NOUVEAU NE; DNA; Sciences bio-médicales et agricoles; DNA, Protozoan; Fetal Blood; Trypanosoma cruzi -- genetics; Infectious Disease Transmission, Vertical; Chagas Disease -- mortality; 3. Good health; HYBRIDATION; Bolivia -- epidemiology; MALADIE DE CHAGAS; POLYMORPHISME GENETIQUE; Chagas Disease -- congenital; ANALYSE GENETIQUE

This study aims to typify the Trypanosoma cruzi (sub)lineage(s) in umbilical cord blood of congenitally infected Bolivian newborns, using PCR amplifications of “Region Markers”, mini-exon or kDNA fragments followed by hybridization or sequencing. New probes were also designed to distinguish three variants within the TcIId sublineage. The IIb, IId, or IIe T. cruzi sublineages, as well as different variants of the IId sublineage, were detected in infected neonates, whereas mixed infections were not found. The frequencies of the IId sublineage were similar in neonates (95.1%) and adults of the same area (94.1%). The IId-infected newborns displayed either asymptomatic, or severe and fatal clinical forms of congenital Chagas disease, as well as low or high parasitemia. Altogether these data show that T. cruzi DNA polymorphism, based on the presently available markers, is not associated with the occurrence of congenital infection or the development of severe clinical forms of congenital Chagas disease.