Multiplex Assay for Species Identification and Monitoring of Insecticide Resistance in Anopheles punctulatus Group Populations of Papua New Guinea

• DOI: 10.4269/ajtmh.2012.11-0503
• Publication Date: 2012-01-09T22:56:40Z
• Authors: Cara N. Henry-Hal...; Kogulan Nadesakum...; John Bosco Keven; Allison M. Zimmerman; Peter Siba; Ivo Mueller; Manuel W. Hetzel; James W. Kazura; Edward Thomsen; Lisa J. Reimer; Peter A. Zimmerman
• Keywords: New guinea; Insecticide Resistance; Multiplex; Identification

Anopheles punctulatus sibling species ( An. s.s., koliensis , and farauti complex [eight cryptic species]) are principal vectors of malaria filariasis in the Southwest Pacific. Given significant effort to reduce transmission through insecticide-treated net distribution region, effective strategies monitor evolution insecticide resistance among An . is essential. Mutations voltage-gated sodium channel VGSC ) gene have been associated with knock-down kdr pyrethroids DDT malarious regions. By examining sequence polymorphism we developed a multiplex assay differentiate wild-type versus alleles query intron-based polymorphisms that enable simultaneous identification. A survey including mosquitoes from seven Papua New Guinea Provinces detected no any species. Absence introgression between evidence geographic separation within suggests must be monitored each independently.