Whole Genome Sequencing of Antibiotic Resistant Genes in Isolates from Surfaces in a Science Laboratory

• DOI: 10.4274/tjps.galenos.2021.29794
• Publication Date: 2022-01-21T08:57:26Z
• Authors: Christiana JESUMI...; Aisha Olamide ABD...; Werner RUPPITSCH
• Keywords: MacConkey agar; Ticarcillin

Isolates obtained from laboratory surfaces were identified and characterized.Ten consecutive isolates 30 sample of a University Science Laboratory in Edo State Nigeria May, 2021. Swabs the aseptically. The swabs streaked on MacConkey, eosin methylene blue, mannitol salt, nutrient agar plates, respectively, incubated appropriately. Distinct colonies randomly culture plates characterized phenotypically. Matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) was used to analyze four (40%) by selection criteria. Susceptibility testing using antibiotics performed for Kirby-Bauer method 15 antibiotics. Isolate characterization identification resistance determinants determined whole genome sequencing (WGS).Microorganisms included Leclercia adecarboxylata, Enterobacter hormaechei, Atlantibacter hermanii, Stenotrophomonas maltophilia. Three antibiotics-resistant investigated WGS. Resistance genes found all (100%) resistant isolates. β-lactamase genes, aminoglycoside modifying enzymes, qnr sulfonamide, tetracycline, trimethoprim respectively. Two carried ESBL blaCTX-M-15 detected.Our study displays dissemination antibiotic among surface Laboratory. To best our knowledge, we have reported first genomic Nigeria.