Comparison of <i>in vitro</i> Resistance of Wild <i>Leishmania</i> İsolates, Which are Resistant to Pentavalent Antimonial Compounds, Against Drugs Used in the Treatment of Leishmaniasis

• DOI: 10.4274/tpd.galenos.2019.6661
• Publication Date: 2020-03-25T08:22:05Z
• Authors: Ahmet Özbilgin; İbrahim Çavuş; Tuğba Kaya; Ahmet Yıldırım; Mehmet Harman
• Keywords: Male; Genotyping Techniques; Turkey; Paromomycin; Phosphorylcholine; Resistance; Antiprotozoal Agents; Drug Resistance; Leishmaniasis, Cutaneous; Infectious and parasitic diseases; RC109-216; Real-Time Polymerase Chain Reaction; resistance; cutaneous leishmaniasis; 03 medical and health sciences; 0302 clinical medicine; Amphotericin B; turkey; Animals; Humans; Leishmania; Cutaneous leishmaniasis; Meglumine Antimoniate; Resistance tests; treatment; R; 3. Good health; Treatment; Antimony Sodium Gluconate; Medicine; Female; resistance tests

InvestigationTurkiye Parazitol Derg 2020;44(1): Objective: Meglumine antimoniate (Glucantime®) and Sodium stibogluconate (Pentostam®) are used for the treatment of cutaneous leismaniasis in Turkey.There is a reported resistance to these drugs recent years.The aim present study was compare vitro sensitivities resistant Leishmania isolates against Amphotericin B, Miltefosine, Antimoniate, Paromomycin Stibogluconate.Methods: Five patients with leishmaniasis, who showed no clinical recovery despite two consecutive meglumine treatments, which were stored Parasite Bank Manisa Celal Bayar University Medical Faculty selected.They genotyped Real-Time PCR using specific primers probes ITS1 region.Drug levels each isolate analysed Paromomycin, Stibogluconate at concentrations 500, 250, 125, 50, 25 µg/mL XTT method hemocytometer.