Extracellular Protease Activity of Enteropathogenic Escherechia coli on Mucin Substrate

• DOI: 10.4308/hjb.14.1.36
• Publication Date: 2017-03-29T19:32:07Z
• Authors: SRI BUDIARTI; NISA RACHMANIA MU...
• Keywords: Enteropathogenic Escherichia coli; Proteolytic enzymes; Yersinia enterocolitica

Enteropathogenic Escherichia coli (EPEC) causes gastrointestinal infections in human. EPEC invasion was initiated by attachment and aggressive colonization on intestinal surface. Attachment of alter the intestine mucosal cells. Despite this, pathogenic mechanism infectior has not been fully understood. This research hypothesizes that extracellular proteolytic enzymes is necessary for colonization. The enzyme secreted into milieu presumably destroy mucus layer cover tract. objective this study to assay protease using mucin substrate. activity 1% substrate investigated. Non-pathogenic E. used as a negative control. Positive tentative controls were Yersinia enterocolitica Salmonella. Ten strains assayed, seven them able degrade mucin, highest produced K1.1 strain. Both positive also showed ability digest 0.20% mucin.