Characterization of Bacteriophage Specific to Bacillus pumilus from Ciapus River in Bogor, West Java, Indonesia

• DOI: 10.4308/hjb.22.1.27
• Publication Date: 2017-03-29T19:36:03Z
• Authors: ANIK KUSMIATUN; IMAN RUSMANA; SRI BUDIARTI
• Keywords: Bacillus pumilus

Bacillus pumilus is a spore-forming bacteria that rod-shaped, gram positive, and aerobic. B. produced pumilacidins, known to have toxic effects on epithelial cells. Antibiotics were usually used treat the disease caused by bacteria. Antibiotic typing test of indigenous from sewage water showed this isolate was resistant ampicillin clindamycin. An alternative way application bacteriophages as biocontrol agents reduce in environment. The aim study characterize bacteriophage isolated Ciapus River Bogor, West Java. Bacteriophages infecting river using double agar overlay method. Phages defined plaque morphology, structure, host range, characteristic molecular weight protein phage. Phage FBa1, FBa2, FBa3 had narrow range they specific for pumilus. Electron microscope observation phage FBa1 icosahedral head without tail (166.67 nm diameter), so it called phage-like particles. Characterization SDS-PAGE five proteins band. Molecular 70.9, 54.9, 33.8, 28.3, 21.4 kDa.