Hepatoprotective perspective of newly synthesized 3-(3,5-bis (tri fluoro methyl) phenyl)- 5-methyl-1-((1-methyl-1h-pyrrol-2-yl) methyl)-2-thioxoimidazolidin-4-one against diethyl nitrosamine induced liver injury in rats with molecular docking investigatio

• DOI: 10.4314/bcse.v38i3.16
• Publication Date: 2024-03-14T11:21:29Z
• Authors: Lana S. Akree; Hiwa O. Ahmad; Zahra A. Amin
• Keywords: Nitrosamine

The hepatoprotective effect of synthesized 3-(3,5-bis (trifluoromethyl) phenyl)-5-methyl-1-((1-methyl-1H-pyrrol-2-yl) methyl)-2-thioxoimidazolidin-4-one (3FL-M) was evaluated against diethyl nitrosamine-induced liver injury (DEN). Wistar rats were divided into 3 groups as: placebo (received 10% tween 80%), hepatotoxic control (injected with 200 mg/kg DEN) and treatment DEN received 50 oral feeding the 3FL-M). Half number sacrificed on 2nd week experiment, whereas other half after 6 weeks. Blood collected to run biochemical analysis, evaluate pro-inflammatory cytokines tumor necrosis factor-alpha TNF-α interleukine6 IL-6. Liver sections used detect nuclear protein ki-67 hepatocyte specific antibody HSA. 3FL-M subjected molecular docking calculations based binding affinities towards DEN-treated showed elevation in serum enzymes as well clear destruction hepatic architecture, contrast treated normalized levels resolution hepatocytes. Molecular modelling revealed that exhibited significant toward pocket IL-6, however, further studies is recommended for developing it a chemotherapeutic drug-like molecule.
 KEY WORDS: DEN, Hepatoprotective, TNF-α, docking
 Bull. Chem. Soc. Ethiop. 2024, 38(3), 751-763. 
 DOI: https://dx.doi.org/10.4314/bcse.v38i3.16