Anti-Snake Venom, Anti-Arthritic and Cytotoxic Activities of <i>Tectona grandis</i> L. f. Stem Bark (Lamiaceae)

• DOI: 10.4314/epj.v35i2.2
• Publication Date: 2020-11-03T14:47:07Z
• Authors: Adebayo Gbolade; Oluwasegun Adedokun; Ogochukwu Ume; Johnkennedy Onyec...; Cynthia Mkparu
• Keywords: Tectona; Combretaceae

Tectona grandis L. f. (Lamiaceae) is famous for timber production and has been used in traditional medicine treating bronchitis, liver-related roubles, urinary discharge amongst other diseases. Methanol extract of the stem bark was investigated vivo antiophidian assay against Bitis arietans Naja nigricollis. Also, chromatographic fractions were subjected to cytotoxicity tests using tadpole model antiarthritic by proteinase inhibition. Chromatography crude methanol afforded three highly polar vacuum liquid chromatography (VLC) (BVLC-1, BVLC-2 BVLC-3). further gave four semi-pure uncharacterized isolates (I, II, III, IV). Only BVLC-3 cytotoxic at 10 - 80 mg/ml, with being most potent (100% mortality, LC50 40 mg/ml). Concentration-dependent enzyme inhibition (24 71%) 200 1000 μg/ml observed, this less (IC50 659.24 μg/ml) than activity standard drug, acetyl salicylic acid. However, neutralization B. N. nigricollis snake venoms not dose-dependent, but atthe least dose, 50 mg/kg offered better protection (75%) on envenomed-mice 48 h. Its comparable that positive antivenin tested 0.2 mg/kg. These findings justify folkloric use T. treatment bites, arthritic conditions, oxidative stress-induced diseases.&#x0D; Keywords: grandis, extract, anti-snake venom activity, cytotoxicity, anti-arthritic