Comparative studies on the antioxidant potential of hydrolysates of <i>Mormyrus rume</i> muscle protein

• DOI: 10.4314/ijs.v21i2.16
• Publication Date: 2019-07-31T10:12:36Z
• Authors: J.A. Jerome; O.O. Odekanyin
• Keywords: Pepsin

This study determined the degree of hydrolysis Mormyrus rume muscle proteins by digestive proteases and antioxidant potential hydrolysates produced was evaluated. The were extracted with distilled water adjusted to neutral pH. used include trypsin, chymotrypsin pepsin using trichloroacetic acid precipitation method. antioxidative activities evaluated different in-vitro assay methods such as 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging, hydrogen peroxide metal chelating capacity reductive potentials. Degree generally low ranges between 17.68% 32.20% given highest hydrolysis. protein obtained trypsin exhibited when assessed activity half maximal inhibitory concentration (IC ) 0.32 ± 0.01 mg/ml; scavenging IC 0.18 mg/ml 50 0.41 0.002 units at tested (1.0 mg/ml). Pepsin-produced showed DPPH all concentrations 0.35 0.003mg/ml. concluded that contain bioactive peptides which possessed significant amount activities, may be a promising health promoting agent if isolated.Keywords: Hydrolysates, Pepsin, Trypsin, Chymotrypsin, Antioxidant, Enzyme, rume.